Product No.：B13350
Size: 50mL
Mechanism of Action
ProClin preservatives attack the central metabolic cycle of the cell, the Krebs cycle, at three specific enzyme sites: α-ketoglutarate dehydrogenase, succinate dehydrogenase, and NADH dehydrogenase. Other inhibited enzymes include pyruvate dehydrogenase, which is vital for the oxidation of pyruvate to acetyl-CoA; as well as lactate dehydrogenase and alcohol dehydrogenase, which detoxify waste by-products synthesized during fermentation. With cellular metabolism debilitated, microorganisms rapidly lose their ability to produce ATP and carry on vital functions, such as transcription, translation and damage repair. All bacteria and fungi possess at least some parts of the Krebs cycle, thus ProClin preservatives represent a highly efficient broad spectrum biocide.
Key Features of ProClin 300
• Compatibility with key enzymes and diagnostic assay indicators
• 3 year shelf life
• Will not inhibit antibody binding
•Safe; environmentally friendly; easy-to-use water-soluble liquid, effective at very low concentrations, typically 0.03 – 0.05% (w/w)
• Excellent stability over a wide pH range
• Stable in TRIS, TES, and HEPES buffer up to a pH of 7
• Level of active ingredients assayable by HPLC
Formulation
These numbers do not constitute specifications.
• 5-Chloro-2-methyl-4-isothiazolin-3-one (CMIT)
• 2-Methyl-4-isothiazolin-3-one (MIT)
• Proprietary glycol: 93 – 95%
• Modified alkyl carboxylate: 2 – 3%
Manufacturing Specifications
• Appearance: clear, colorless to light yellow liquid, free of foreign matter
• Gardner Color: 3 maximum
• Total Active Ingredients: 3.00 – 3.60%
• 5-Chloro-2-methyl-4-isothiazolin-3-one: 2.10 – 2.80%
• 2-Methyl-4-isothiazolin-3-one: 0.60 – 1.00%