HHBS [Hanks’ Buffer with 20 mM Hepes]
Cat No.: C00200
Package: 500ml
Storage: Store at 4 °C. Expiration date is one year from the date of receipt.
Product Description
Hank's Balanced Salt Solution with 20 mM Hepes buffer (HHBS) is used for a variety of cell culture applications, such as washing cells, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation such as calcium, membrane potential assay buffer preparation.
Compositions：
COMPONENTS | Molecular Weight | Concentration (mg/L) | mM |
Inorganic Salts | |||
Calcium Chloride (CaCl2) (anhyd.) | 111 | 140 | 1.26 |
Magnesium Chloride (MgCl2-6H2O) | 203 | 100 | 0.49 |
Magnesium Sulfate (MgSO4-7H2O) | 246 | 100 | 0.41 |
Potassium Chloride (KCl) | 75 | 400 | 5.33 |
Potassium Phosphate monobasic (KH2PO4) | 136 | 60 | 0.44 |
Sodium Bicarbonate (NaHCO3) | 84 | 350 | 4.17 |
Sodium Chloride (NaCl) | 58 | 8000 | 137.93 |
Sodium Phosphate dibasic (Na2HPO4) anhydrous | 142 | 48 | 0.34 |
Other Components | |||
D-Glucose (Dextrose) | 180 | 1000 | 5.56 |
Hepes | 238 | 4760 | 20 |