1,4-Dithio-DL-threitol （DTT）
Product Number: E00580
CAS Number: 3483-12-3
Appearance：White Powder
Molecular formula: C4H10O2S2
Molecular weight：154.25 g/mol
Solubility：0.1 M in H2O, 20℃, complete, colorless
Storage Temperature: 2-8℃
DTT is synthetic. A method of preparation has been described in J. Chem. Soc. 253 (1949).
DTT is an agent for reducing -S-S- to -SH. Redox potential: -0.33 volts at pH 7. DTT acts as a protective agent for -SH groups of other species in solution. In this reaction the DTT is oxidized to the cyclic disulfide, and thereby ensures the reduction of other disulfides in solution. The disulfide reduction is complete in minutes at pH 8. A specific and sensitive assay for disulfide groups is based on determination of resulting monothiols with DTNB.
Applications:
It is used in buffers for isolation of RNA or buffers used for RT-PCR starting with mRNA.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.
Preparation Instructions:
The solubility of DTT has been tested by Fluka at 50 mg/ml in water, resulting in a clear colorless solution. It has been described as freely soluble in water, ethanol, acetone, ethylate, chloroform and ether. Solutions oxidize relatively slowly in air. It is recommended to make solutions fresh daily.