β-Nicotinamide adenine dinucleotide, reduced disodium salt
Catalog Numbers: E03760
Size: 250mg/1g
Synonyms: β-NADH, NADH, β-DPNH, DPNH, Diphosphopyridine nucleotide, reduced form
CAS: 606-68-8
Molecular Formula: C21H27N7Na2O14P2
Molecular Weight: 709.4
Purity: >98%
Appearance: White to Light Yellow Powder
Storage Temperature –20℃. b-NADH should be stored desiccated and protected from light.
β-NADH, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. β-NAD+ is a carrier for hydride ion, forming β-NADH. The hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as, malic dehydrogenase and lactic dehydrogenase. These enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to β-NAD +, forming the reduced product, β-NADH. Unlike β-NAD+ which has no absorbance at 340 nm, β-NADH absorbs at 340 nm. The increase in absorbance (with the formation of β-NADH) or the decrease in absorbance (with the formation β-NAD+) is the basis for measurement of activity of many enzymes at 340 nm.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NADH as a cofactor and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. β-NADH is fluorescent; whereas, β-NAD+ is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Preparation Instructions
This product is soluble in 0.01 M NaOH (100 mg/ml). Water alone should not be used to prepare solutions since it tends to be acidic and would decompose β-NADH. If solutions must be stored for any length of time, phosphate buffers should be avoided since they accelerate the destruction of β-NADH. Trizma(0.01 M, pH 8.5) and MES buffers are better options. Since β-NADH solutions are susceptible to oxidation even at low temperatures, solutions should be prepared at concentrations no greater than 5mM, at a pH of 9–11, and stored at 4℃.
The presence of light and heavy metals can accelerate the oxidation process. If a low temperature freezer is available (temperatures at –40℃ or colder), more concentrated solutions can be prepared and stored for years without any loss of activity.
Precautions and Disclaimer:
For Laboratory Use Only. Not for drug, household or other uses.