Glutathione Reductase from baker's yeast
Product Number: E03950
Enzymatic Activity: 100-300 units/mg protein
Storage Temperature: 2-8 ° C
Product Description
Enzyme Commission (EC) Number: 1.6.4.2
CAS Number: 9001-48-3
Molecular Weight: 124 kDa
Glutathione reductase from Baker's yeast is a flavoprotein homodimer consisting of two equal subunits. Each subunit has one mole of FAD which is noncovalently bound. Glutathione reductase is also a sulfhydryl protein containing a total of six sulfhydryl groups.The enzyme catalyzes the following reaction:
Glutathione(Oxidized) + β-NADPH →β-NADP + 2 Glutathione(Reduced)
The Km values for the enzyme are: oxidized glutathione (61 μM) and β -NADPH (7.6 μm).
Gltathione reductase is inhibited by the following inhibitors:
N-alkylmaleimides; benzylselenosulphate; 2-chloroethylisocyanate; Cu2+; 2,4-dihydroxybenzylamine; 1-fluoro-2,4-dinitrobenzene; p-nitrobenzylselenosulphate ; 2-triazine-5-nitrofuran
Unit Definition
One unit will reduce 1.0 μmole of oxidized glutathione per min at pH 7.6 at 25 °C.
Physical form
Suspension in 3.6 M (NH4)2SO4, pH 7.0, containing 0.1mM dithiothreitol
Precautions and Disclaimer
For Laboratory Use Only. Not for drug, household or other uses.
Storage/Stability
This product is offered as an ammonium sulfate suspension. Diluted stock solutions should not be prepared.