无氨基酵母氮源 (YNB)（含硫酸铵）说明书
货号：W00030
规格：100g /500g
保存：RT，有效期5年。
产品简介：
氨基酸与碳水化合物需求基础上酵母分类。
YNB培养基成分：
Nitrogen Source
Ammonium Sulfate.................................... 5.0g
Vitamins
Biotin........................................................ 2.0μg
Calcium Pantothenate .............................. 400.0μg
Folic Acid ................................................. 2.0μg
Inositol ...................................................2,000.0μg
Niacin .......................................................400.0μg
p-Aminobenzoic Acid .............................. 200.0μg
Pyridoxine Hydrochloride ......................... 400.0μg
Riboflavin ...................................................200.0μg
Thiamine Hydrochloride .............................400.0μg
Compounds Supplying Trace Elements
Boric Acid ...................................................500.0μg
Copper Sulfate............................................... 40.0μg
Potassium Iodide............................................ 100.0μg
Ferric Chloride ............................................. 200.0μg
Sodium Molybdate...................................... 200.0μg
Zinc Sulfate .................................................400.0μg
Salts
Monopotassium Phosphate ............................... 1.0 g
Magnesium Sulfate ........................................... 0.5 g
Sodium Chloride ................................................0.1 g
Calcium Chloride............................................... 0.1 g
YNB培养基配置:（仅供参考）
0.67% (single strength) or 6.7% (10×) solution, soluble in purified water with agitation. Single-strength solution is colorless to very pale yellow and clear; 10× solution is yellow and clear. Prepared Appearance: Colorless, clear. Reaction of 0.67% Solution at 25°C: pH 5.4 ± 0.2
注意事项:
- YNB培养基的灭菌方法是用滤器除菌，不能用高压灭菌。凡是具有生物活性的物质，要保持生物活性一般都不能用高压灭菌。生物素及组氨酸溶液也只能用滤器除菌。
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